Nostalgia
发表于 2025-3-25 03:28:02
Mechanisms of Protein Trafficking as precursor proteins. That is, they have an extension of amino acids at their N-terminal end. This extension is removed after import by the action of a specific protease. Thus, when a mitochondrial protein was isolated, the extension, called a leader sequence, was missing from most, but not all, m
不在灌木丛中
发表于 2025-3-25 11:26:04
Analysis of Nucleocytoplasmic Transport Using Green Fluorescent Proteinparation introduces a potent and sophisticated level of regulation, it also requires highly effective and selective transport machinery. All known transport between the nucleus and the cytoplasm occurs through the nuclear pore complex (.–.). Theoretically, proteins<40 kDa can enter and leave the nuc
machination
发表于 2025-3-25 14:21:18
Transgenic Bovine Embryo Selection Using Green Fluorescent Proteinn, receptor mediated gene transfer, sperm vector, the combination of sperm vector and intracytoplasmic sperm injection and nuclear transplantation have been successfully used to produce transgenic animals (.–.). However, low effciency and high mosaic rates are still major problems for most gene deli
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发表于 2025-3-25 18:45:46
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一加就喷出
发表于 2025-3-25 21:02:20
New Strategies for Financial Services Firmsr the plasmid pEB1, for the overexpression of a truncated . gene of ., exhibit bright red fluorescence .) when cultured on Luria-Bertani (LB) growth medium and illuminated with ultraviolet (UV) light. The genes both encode uroporphyrinogen III (urogen III) methyltransferases (referred herein to as C
凹处
发表于 2025-3-26 00:38:20
P. J. A. Burt,J. Colvin,S. M. Smithng in intact cells and organisms (.–.). GFP represents the first genetically encoded reporter molecule that is detectable in the absence of an enzymatic substrate or cofactor, in a variety of cell types.
喃喃诉苦
发表于 2025-3-26 08:03:21
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我没有强迫
发表于 2025-3-26 10:00:13
https://doi.org/10.1007/978-1-349-02396-7iving cells (.). In most cases, GFPs were added at either the C- or Nterminal end of the protein or polypeptide of interest (.). For certain purposes, sueh as fluorescence resonance energy transfer (FRET), GFPs need to be placed at particular locations within the protein (.). Because the crystal str
结果
发表于 2025-3-26 15:10:52
https://doi.org/10.1007/978-1-349-02399-8combinant systems. To facilitate recombinant production of such proteins for structural and engineering studies, the author has developed a method for producing messenger RNAs on circular RNA templates. This circularization process is derived from a rearranged group I intron, from which circular RNA
inspired
发表于 2025-3-26 19:05:15
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