表主动
发表于 2025-3-25 06:48:37
http://reply.papertrans.cn/39/3871/387057/387057_21.png
Amplify
发表于 2025-3-25 10:04:45
https://doi.org/10.1007/978-3-031-28722-0embranes and cytosol, synapses, and other intermediate cellular membranes from postmortem human brain tissue. These subcellular fractions can be used in a variety of downstream applications to assess the localization, relative abundance, and stoichiometry of glutamate receptor subunits along the forward trafficking pathway.
软膏
发表于 2025-3-25 11:56:19
http://reply.papertrans.cn/39/3871/387057/387057_23.png
懒洋洋
发表于 2025-3-25 17:23:13
http://reply.papertrans.cn/39/3871/387057/387057_24.png
Pudendal-Nerve
发表于 2025-3-25 22:45:01
http://reply.papertrans.cn/39/3871/387057/387057_25.png
exclusice
发表于 2025-3-26 03:50:54
http://reply.papertrans.cn/39/3871/387057/387057_26.png
万神殿
发表于 2025-3-26 05:41:47
Commodification and the Market,us Percoll-sucrose density gradients, collection of brain tissue, preparation of brain homogenates, isolation of synaptoneurosome bands from the discontinuous Percoll-sucrose gradients, and radiolabeling SN proteins. De novo protein synthesis can be reproducibly measured in SN prepared by this method.
enumaerate
发表于 2025-3-26 10:43:02
Electrophysiological Investigation of Metabotropic Glutamate Receptor-Dependent Metaplasticity in thicity in acute hippocampal slices. While this chapter focuses on in vitro field electrophysiological investigations, many of the principles can be applied to single-cell recordings as well as in vivo electrophysiology and indeed many types of metaplasticity phenomena.
修饰
发表于 2025-3-26 13:45:22
Whole-Cell Patch-Clamp Electrophysiology to Study Ionotropic Glutamatergic Receptors and Their Roles) and N-methyl-.-aspartate (NMDA) receptors underlying behavior has been extensively studied. Here we will discuss commonly used protocols and techniques for performing whole-cell patch-clamp recordings and exploring AMPA and NMDA receptor-mediated glutamatergic responses and alterations in the context of substance abuse.
讽刺
发表于 2025-3-26 19:47:11
Gene Expression Analysis by Multiplex Single-Cell RT-PCRerase chain reaction (PCR), how to design gene-specific probes, and how to validate them. This technique provides multiplexed expression data that cannot be easily obtained by other approaches such as immunological co-labeling.