GIBE
发表于 2025-3-25 14:38:36
Stefan Zielonka,Simon KrahIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
吗啡
发表于 2025-3-25 18:04:04
Isolation of Antigen-Specific Unconventional Bovine Ultra-Long CDR3H Antibodies Using Cattle Immuniial of bovine-derived antigen-specific ultra-long CDR3 antibodies, a straightforward and effective high-throughput method based on yeast surface display and fluorescence-activated cell sorting is described.
变化
发表于 2025-3-25 20:03:58
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注入
发表于 2025-3-26 04:10:34
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Etymology
发表于 2025-3-26 06:04:11
https://doi.org/10.1007/978-3-642-91574-1in one antibody. We present the strategies for construction and selection of yeast libraries displaying heterodimeric Fcab fragments and discuss the effects of altered thermostability of the basic Fc scaffold and novel library designs that lead to isolation of highly affine antigen binding clones.
障碍
发表于 2025-3-26 09:15:33
https://doi.org/10.1007/978-3-662-33292-4n of cognate VH-VL sequences, compatible with both next-generation sequencing (NGS) and YSD library cloning. We employ a single B cell encapsulation in water-in-oil droplets, followed by a one-pot reverse transcription overlap extension PCR (RT-OE-PCR), resulting in a paired VH-VL repertoire from more than a million B cells in a single day.
lymphoma
发表于 2025-3-26 14:58:20
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冷淡周边
发表于 2025-3-26 18:27:04
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伸展
发表于 2025-3-26 22:41:58
Selection of High-Affinity Heterodimeric Antigen-Binding Fc Fragments from a Large Yeast Display Liin one antibody. We present the strategies for construction and selection of yeast libraries displaying heterodimeric Fcab fragments and discuss the effects of altered thermostability of the basic Fc scaffold and novel library designs that lead to isolation of highly affine antigen binding clones.
Lipoma
发表于 2025-3-27 04:12:29
One-Pot Droplet RT-OE-PCR for the Generation of Natively Paired Antibody Immune Libraries,n of cognate VH-VL sequences, compatible with both next-generation sequencing (NGS) and YSD library cloning. We employ a single B cell encapsulation in water-in-oil droplets, followed by a one-pot reverse transcription overlap extension PCR (RT-OE-PCR), resulting in a paired VH-VL repertoire from more than a million B cells in a single day.