Disclose 发表于 2025-3-21 18:24:07
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Dicentric Chromosome Assay (DCA) and Cytokinesis-Block Micronucleus (CBMN) Assay in the Field of Biomes (Dic) and micronuclei (MN) in exposed individuals’ peripheral blood lymphocytes (PBL)..The present chapter does not claim to make an exhaustive and complete picture on the complex world of biodosimetry, to which a large number of specific guidelines for performing laboratory services by the Inte落叶剂 发表于 2025-3-22 08:37:00
Micronucleus Cytome Assays in Human Lymphocytes and Buccal Cellsrs to the use of ex vivo whole blood method, involving 72 h of culture with the block of cytokinesis starting at 44 h. BMNCyt protocol reports the established method for sample collection, processing, slide preparation and scoring.津贴 发表于 2025-3-22 12:03:49
Micronucleus Analysis by Flow Cytometry of the in vitro MN test Organization for Economic Cooperation and Development (OECD) guideline 487 (OECD, Guideline for testing of chemicals: in vitro mammalian cell micronucleus test 487: in vitro mammalian cell micronucleus test (MNVIT). Organization for Economic Cooperation and Development, Pari定点 发表于 2025-3-22 16:36:43
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The Comet Assay in Human Biomonitoringder to remove all cellular contents except the DNA attached to a nuclear scaffold. Subsequent electrophoresis results in accumulation of undamaged DNA sequences at the proximity of the nuclear scaffold, while damaged sequences migrate toward the anode. When visualized with fluorochromes, these migraFECK 发表于 2025-3-23 03:54:48
Multicolor Laser Scanning Confocal Immunofluorescence Microscopy of DNA Damage Response Biomarkersing confocal immunofluorescence microscopy (LSCIM) of three-dimensionally preserved nuclei can be, quantitative and maintain the spatial information. Here we describe the experimental protocols to quantify individual key events of the DDR cascade in three-dimensionally preserved nuclei by LSCIM withProcesses 发表于 2025-3-23 06:13:37
Detection of DNA Double-Strand Breaks Using Pulsed-Field Gel Electrophoresislly relevant cellular processes as well as to investigate the genotoxic or clastogenic effects of toxicants. Pulsed-field gel electrophoresis (PFGE) is a widely used method for direct quantification of DNA DSBs. In this method, the cells exposed to DSB-inducing agents are embedded in the agarose blo