可卡
发表于 2025-3-26 23:44:57
https://doi.org/10.1007/978-3-8349-8779-2ryonic stem cells, an exogenous DNA sequence can be inserted into the target locus in the zygote using genome-editing technology. In this chapter, I describe the generation of epitope-tagged mice using engineered endonuclease and single-strand oligodeoxynucleotide through the mouse zygote as an exam
取之不竭
发表于 2025-3-27 01:14:13
https://doi.org/10.1007/978-3-8349-4748-2nding disease pathophysiology. Recently, important advances in genome editing technologies have enabled us to efficiently create sophisticated animal models in short periods of time. Base editing is a modified CRISPR/Cas system that induces base substitution at targeted genomic regions. Here I descr
gentle
发表于 2025-3-27 06:41:13
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改正
发表于 2025-3-27 11:44:48
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联合
发表于 2025-3-27 15:20:11
https://doi.org/10.1007/978-3-031-21274-1tegies for genotyping flox mice, typically based on Sanger sequencing following cloning of target sequences from dozens of pups, are time-consuming. Here, we describe a rapid screening method for flox mice, using in vitro Cre recombination that can be performed using simple enzymatic reactions and e
爱国者
发表于 2025-3-27 21:46:42
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Memorial
发表于 2025-3-27 21:58:09
Professionelles Desktop Publishing, after the Cas9-induced double-strand DNA breaks; the activation of the DNA repair pathway is known to be correlated with the cell cycle. Recently, we have reported a new KI approach named SPRINT (.-phase .onuclear .jection for .argeting)-CRISPR, focusing on the correlation between the cell cycle an
Homocystinuria
发表于 2025-3-28 05:57:33
Desktop Publishing — Was bringt’s wirklich?s. The intravenous injection of an AAV vector harboring the gene of interest driven by the hepatocyte-specific promoter could efficiently express the target gene in liver hepatocytes. The delivery of genome editing tools including Cas9 and gRNA, by the AAV vector, can efficiently disrupt the target
Encoding
发表于 2025-3-28 07:40:27
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和蔼
发表于 2025-3-28 12:21:11
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