anachronistic 发表于 2025-3-23 10:19:32
http://reply.papertrans.cn/39/3829/382836/382836_11.pngBlood-Clot 发表于 2025-3-23 17:33:40
http://reply.papertrans.cn/39/3829/382836/382836_12.pngApogee 发表于 2025-3-23 18:27:41
Efficient CRISPR/Cas9-Assisted Knockin of Large DNA Donors by Pronuclear Microinjection During S-Ph after the Cas9-induced double-strand DNA breaks; the activation of the DNA repair pathway is known to be correlated with the cell cycle. Recently, we have reported a new KI approach named SPRINT (.-phase .onuclear .jection for .argeting)-CRISPR, focusing on the correlation between the cell cycle anapiary 发表于 2025-3-24 01:21:36
Genome Editing of Murine Liver Hepatocytes by AAV Vector-Mediated Expression of Cas9 In Vivo,s. The intravenous injection of an AAV vector harboring the gene of interest driven by the hepatocyte-specific promoter could efficiently express the target gene in liver hepatocytes. The delivery of genome editing tools including Cas9 and gRNA, by the AAV vector, can efficiently disrupt the target抛射物 发表于 2025-3-24 06:02:16
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http://reply.papertrans.cn/39/3829/382836/382836_16.png完全 发表于 2025-3-24 11:32:04
A Simple and Efficient Method for Generating KO Rats Using In Vitro Fertilized Oocytes,ce. However, in rats, an efficient genome editing technique that uses in vitro fertilized oocytes has not been established. Recently, we reported the stable generation of offspring from five standard strains of rats by superovulation and in vitro fertilization (IVF). Furthermore, genome-edited ratspromote 发表于 2025-3-24 16:53:36
Editing the Genome of the Golden Hamster (,),antageous in the study of reproductive and developmental biology: a highly stable 4-day estrous cycle, a high responsiveness to conventional superovulation methods, and a shortest gestation period (16 days) known among eutherian mammals. Besides these advantages, the technical ease of in vitro ferti手段 发表于 2025-3-24 20:14:16
http://reply.papertrans.cn/39/3829/382836/382836_19.pngDEI 发表于 2025-3-25 02:08:59
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