mortuary
发表于 2025-3-28 18:02:11
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Scleroderma
发表于 2025-3-28 22:45:53
Conversations with a Mathematicianlogy, is a very useful method for studying gene expression particularly when handling limited amounts of starting materials (. .). Using this method, analysis of gene expression can be carried out at a single-cell level (.–.). This is not possible using conventional techniques of mRNA isolation and cDNA cloning.
迅速成长
发表于 2025-3-28 23:52:24
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instill
发表于 2025-3-29 03:50:05
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Heterodoxy
发表于 2025-3-29 10:56:22
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larder
发表于 2025-3-29 15:09:46
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quiet-sleep
发表于 2025-3-29 19:08:20
1064-3745 n extensively utilized to identify numerous gene probes in vitro and in vivo. However, attempts to generate complete and full-length complementary cDNA libraries were, for the most part, fruitless and remained elusive until the last decade, when simple and rapid methods were developed. With current
Pessary
发表于 2025-3-29 21:49:28
https://doi.org/10.1057/9780230378827nd and an adaptor at the 5′ end are added to the first strand of cDNA during reverse transcription; amplification of virtually any transcript to either end can then make use of this same pool of cDNAs. In addition to being simple, the efficiency of 5′-RACE is dramatically increased because the adaptor is added only to full-length cDNAs.
RAG
发表于 2025-3-30 02:00:07
https://doi.org/10.1007/978-3-658-38509-5es of mRNAs from a limited amount of promoter-linked cDNAs (.,.) (. .). However, the aRNAs prepared from a single live neuron has been reported to cover 50–75% of the total mRNA population (.,.), indicating that rare mRNAs were lost during the amplification procedure.
Femine
发表于 2025-3-30 04:53:34
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