祈求
发表于 2025-3-21 17:22:04
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amputation
发表于 2025-3-21 22:53:29
Identifying Essential , Genes Using Genome-Wide Deletion Mutation,enes in the . SK36 strain using genome-wide deletion mutation. A fusion PCR-based method is used to construct gene deletion fragments, which contain kanamycin resistance cassettes with two flanking arms of DNA upstream and downstream of the target gene. The linear fused PCR amplicons were transforme
ARBOR
发表于 2025-3-22 02:14:05
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NUL
发表于 2025-3-22 06:21:37
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纯朴
发表于 2025-3-22 10:26:40
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GEAR
发表于 2025-3-22 13:12:25
Identifying Essential Genes in , by Global Phenotypic Profiling,libraries after pressure from selection. Although this method can be used for any organism in which transposon mutagenesis is possible, this chapter describes its use in .. More specifically, the methods for generating complex libraries through transposon mutagenesis, design of selective pressure, e
GEAR
发表于 2025-3-22 18:39:04
Essential Genes in the Infection Model of ,-PCR-Based Signature-Tagged Mutagenesis,tion of genes that will diminish or enhance maintenance of an organism in a specific ecological niche or environment. PCR-based STM applied to . permitted the identification of genes essential for in vivo maintenance by transposon insertion and negative selection in a mixed population of bacterial m
爱了吗
发表于 2025-3-23 00:14:29
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LAP
发表于 2025-3-23 02:04:53
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FLOUR
发表于 2025-3-23 06:38:17
A Statistical Framework for Improving Genomic Annotations of Transposon Mutagenesis (TM) Assigned Eiment to identify essential genes in Prokaryota. However, due to the limitation of high-throughput technique, this approach yields substantial systematic biases resulting in the incorrect assignments of many essential genes. To obtain unbiased and accurate annotations of essential genes from TM expe