暖昧关系
发表于 2025-3-25 05:32:33
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薄荷醇
发表于 2025-3-25 07:34:39
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键琴
发表于 2025-3-25 12:22:15
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失眠症
发表于 2025-3-25 19:00:49
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ABOUT
发表于 2025-3-25 23:38:28
Measuring GPCR Stoichiometry Using Types-1, -2, and -3 Bioluminescence Resonance Energy Transfer-Bas interactions from non-specific associations that inevitably result from constraining proteins in cellular membranes. In our experience, concordant data obtained in two or more of these assays, benchmarked with suitable controls, strongly predict receptor stoichiometry.
柱廊
发表于 2025-3-26 02:44:32
Quantification and Comparison of Signals Generated by Different FRET-Based cAMP Reportersthe underlying cAMP change. In this chapter, we provide examples of the problems that may arise when using normalized FRET data and present a method based on the conversion of FRET ratio changes into actual cAMP concentrations that mitigates these issues.
heckle
发表于 2025-3-26 08:09:13
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山羊
发表于 2025-3-26 09:36:23
Transradial Approach for Strokeies do not discriminate ligand binding on GPCR monomers, homo- or hetero-oligomers. To address this issue, we recently developed a new assay based on a time-resolved FRET method that is easy to implement and that can focus on ligand binding specifically on the hetero-oligomer.
FLAT
发表于 2025-3-26 14:25:28
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Hippocampus
发表于 2025-3-26 20:34:02
Molecular Dynamic Simulations to Probe Water Permeation Pathways of GPCRsthe MD simulations of the rhodopsin . The data analysis includes the root mean square deviation (RMSD) and mapping of accessibility of water molecules.