放肆的你
发表于 2025-3-26 22:01:22
Quantifying GPCR Internalization: A Focus on the Kisspeptin Receptorhe use of immunofluorescence and imaging techniques as well as flow cytometry. The techniques described use the FLAG-tagged kisspeptin receptor (KISS1R) as an example but are equally applicable to any other GPCR.
Provenance
发表于 2025-3-27 01:16:13
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喃喃诉苦
发表于 2025-3-27 05:52:17
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Seizure
发表于 2025-3-27 13:30:56
Measurement of Surface-Mediated Ca2+ Transients on the Single-Cell Level in a Microfluidic Lab-on-a-er cells or particles, and the simultaneous analysis of the induced cytosolic calcium signals. The method is based on a microfluidic lab-on-a-chip environment that allows for contactless cell and particle handling by dielectrophoretic forces.
增强
发表于 2025-3-27 14:09:15
https://doi.org/10.1007/978-3-0348-6378-0luorescence plate reader platforms. For such assays a considerable amount of cells expressing the desired biosensor is needed. A method to achieve sufficient and reproducible level of cAMP biosensor protein expression with the means of BacMam transduction system is the subject of this chapter.
modest
发表于 2025-3-27 18:13:07
cAMP Assay for GPCR Ligand Characterization: Application of BacMam Expression Systemluorescence plate reader platforms. For such assays a considerable amount of cells expressing the desired biosensor is needed. A method to achieve sufficient and reproducible level of cAMP biosensor protein expression with the means of BacMam transduction system is the subject of this chapter.
割公牛膨胀
发表于 2025-3-27 22:34:27
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crescendo
发表于 2025-3-28 05:34:36
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独特性
发表于 2025-3-28 10:08:28
https://doi.org/10.1007/978-3-658-24813-0 activity integrates this complexity. In this assay protocol, we describe how the xCELLigence RTCA HT impedance-based platform which can be used for functional cell-based GPCR assays can be utilized for GPCR screening.
Minutes
发表于 2025-3-28 13:54:25
https://doi.org/10.1007/978-3-658-01505-3gand-directed desensitization of the receptor. DMR antagonist reverse assays manifest biased antagonism. DMR profiling using distinct probe-modulated cells detects the biased agonism in the context of self-referenced pharmacological activity map.