commensurate
发表于 2025-3-26 21:20:23
https://doi.org/10.1007/978-3-531-90655-3quality of starting material. Here we describe the preparation and isolation of epizootic hemorrhagic disease virus (EHDV) from blood and tissue samples in tissue culture flasks (TCFs) through the inoculation of susceptible cell lines including Vero, BHK, and KC cells.
dominant
发表于 2025-3-27 02:43:18
https://doi.org/10.1007/978-3-662-67801-5d confirmation of disease. Several real-time RT-PCR methods have been reported over the last 10 years. In this chapter, we describe seven duplex real-time RT-PCR assays to amplify part of genome segment 2 of EHDV to enable serotype identification. The assay includes the detection of an endogenous control gene—beta-actin.
Daily-Value
发表于 2025-3-27 07:19:59
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长处
发表于 2025-3-27 09:44:59
Real-Time RT-PCR Assays for Typing of Epizootic Hemorrhagic Disease Virus,d confirmation of disease. Several real-time RT-PCR methods have been reported over the last 10 years. In this chapter, we describe seven duplex real-time RT-PCR assays to amplify part of genome segment 2 of EHDV to enable serotype identification. The assay includes the detection of an endogenous control gene—beta-actin.
Multiple
发表于 2025-3-27 13:57:30
Managing Risk When Working with Orbiviruses with a Special Emphasis on Epizootic Hemorrhagic Diseassessment provides stepwise guidance for completing a risk assessment, from information gathering and identifying hazards to evaluating the risks, developing, and implementing controls and review..To support the development of a mature safety culture within laboratories, it is important that all staf
PRISE
发表于 2025-3-27 18:43:21
Recognition of Field Signs, Necropsy Procedures, and Evaluation of Macroscopic Lesions of Cervids Iong ruminant hosts and . species vectors. A variety of domestic and wild ruminant species are susceptible to EHDV infection, but infection outcome is highly variable between species, as well as between individuals of the same species. Thus, this disease system inherently operates at the wildlife-liv
先锋派
发表于 2025-3-27 23:24:26
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perpetual
发表于 2025-3-28 02:52:16
Purification of Epizootic Hemorrhagic Disease Virus (and Other Orbiviruses) Particles from Infectedosts EHDV, like bluetongue virus (BTV), it has mainly been associated with infection of endothelial cells of capillaries as well as leukocyte subsets. Furthermore, EHDV infects and replicates within its biological vector, . biting midges and .derived cells. A wide range of common laboratory cell lin
myopia
发表于 2025-3-28 07:19:57
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工作
发表于 2025-3-28 10:45:11
Epizootic Hemorrhagic Disease Virus Titration,ral component of epizootic hemorrhagic disease virus (EHDV) research, including estimating infectivity, calculating multiplicity of infection, and confirming virus propagation in cell culture. However, the ability to quantify infectious EHDV is also critical for disease control, particularly in the