充裕
发表于 2025-3-21 16:27:02
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TIA742
发表于 2025-3-21 23:30:16
Strategies for Undertaking Expressed Sequence Tag (EST) Projects,rposes. They are especially important for enhancing the utility of a genome sequence or for providing a gene catalog for a genome that has not or will not be sequenced. In planning and executing a cDNA project, several criteria must be considered. One should clearly define the project purpose, inclu
ascend
发表于 2025-3-22 02:55:50
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蒸发
发表于 2025-3-22 05:32:44
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CLAY
发表于 2025-3-22 11:03:41
Preparation of Full-Length cDNA Libraries: Focus on Metazoans,btraction of redundant transcripts. The use of trehalose and sorbiol stabilizes the activity of the reverse transcriptase leading to efficient cDNA synthesis and the cap-trapping method is used for efficient full-length cDNA selection. Through the incorporation of additional normalization and subtra
出来
发表于 2025-3-22 14:46:34
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出来
发表于 2025-3-22 17:25:02
Generating EST Libraries: ,-Spliced cDNAs,r a fraction) of their mRNAs. This feature may be exploited for the preparation of full-length-enriched cDNA libraries from these organisms (a diverse group including euglenozoa and dinoflagellates, as well as members from five metazoan phyla: Cnidaria, Rotifera, Nematoda, Platyhelminths and Chordat
CANDY
发表于 2025-3-23 00:59:33
Large-Scale Sequencing and Analytical Processing of ESTs,, their multipurpose uses, such as in probe design for microarrays, determining alternative splicing, verifying open reading frames, and confirming exon/intron and gene boundaries, to name a few, further justify their inclusion in many genomic characterization projects. Hence, there has been a const
liaison
发表于 2025-3-23 02:58:53
EST Processing: From Trace to Sequence,bjects. Here we describe the usage of a software pipeline (available from .), which has been developed to make the most of EST datasets. This modular software solution is targeted toward small- to medium-sized EST projects and comprises a series of Perl scripts. The software design is based on our e
阐释
发表于 2025-3-23 09:27:44
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