filicide
发表于 2025-3-26 22:51:33
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轻弹
发表于 2025-3-27 04:43:21
Competitive Binding Assay for the G-Protein-Coupled Receptor 30 (GPR30) or G-Protein-Coupled Estroges are essential to elucidate the underlying effects of this novel estrogen metabolite and to validate its targets; therefore, this competitive receptor-binding assay protocol was developed in order to assess the membrane receptor binding and affinity of 2-methyoxyestradiol.
不知疲倦
发表于 2025-3-27 06:09:40
Shotgun Proteomics Analysis of Estrogen Effects in the Uterus Using Two-Dimensional Liquid Chromato rat uterus after estrogen (ethinylestradiol) treatment. The steps of the protocol involve sample preparation (digestion), 2D-nanoLC-MS/MS analysis, and shotgun proteomics analysis including bioinformatics tools for data conversion, organization, and interpretation.
毁坏
发表于 2025-3-27 12:46:26
Application of Circular Dichroism Spectroscopy to the Analysis of the Interaction Between the Estroly structured in α-helix, is a key coactivator for ERα activity. Here, we show how circular dichroism can be used to study the interaction of ERα with Ca.-calmodulin. Our approach allows the determination not only of the conformational changes induced upon complex formation but also the dissociation constant (.) of this interaction.
AGONY
发表于 2025-3-27 14:03:20
Book 2016n this book range from standard methods and vital laboratory workhorses, such as receptor binding assays and western blot, to newer technologies such as RNAseq and proximity ligation assay. Chapters also discuss protocols from a broad range of tissue types to demonstrate the variety of estrogen rece
abolish
发表于 2025-3-27 18:10:40
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Sad570
发表于 2025-3-27 22:40:02
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CIS
发表于 2025-3-28 02:27:19
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神圣在玷污
发表于 2025-3-28 06:56:07
Chromatin Immunoprecipitation with Estrogen Receptor 1 and the Promoter of , in TM4 Sertoli Cells,hich is a known estrogen-responsive gene. ChIP results showed the physical interaction between estrogen receptor I (ESR1) and EREs in the . promoter in TM4 mouse Sertoli cells. This chapter describes the protocol for chromatin immunoprecipitation applied to the estrogen response elements in the . promoter.
竖琴
发表于 2025-3-28 10:52:24
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