Glossy
发表于 2025-3-30 12:17:26
Chung-Hua Shen,Qi Liang,Xiang-Chao Haot. During the past decade, considerable progress has been made on the development of flow cytometry (FCM) and fluorescence-activated cell sorting (FACS) methods for the analysis and isolation of both murine and human erythroid cells at distinct stages of erythropoiesis, based on changes in the expre
说笑
发表于 2025-3-30 16:24:39
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Pcos971
发表于 2025-3-30 18:52:50
reticulocyte. Herein, we describe the flow cytometry-based assays for enucleation assessment. The separation of nucleated erythroblasts, reticulocytes, and extruded nuclei by flow cytometry is based on DNA staining, surface expression of erythrocyte specific markers, or forward scatter (FSC). The en
树上结蜜糖
发表于 2025-3-30 21:45:05
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不舒服
发表于 2025-3-31 04:16:51
Jerry McBeath,Jenifer Huang McBeathNA and protein with bi-functional reagents such as formaldehyde and precipitation of the protein with a specific antibody permit PCR amplification (ChIP) or sequencing (ChIP-seq) to identify the bound sites. Here, we present methodology for these approaches that are widely applicable to erythroid ce