无能性 发表于 2025-3-28 14:57:08

Comprehensive and Comparative Structural Glycome Analysis in Mouse Epiblast-like Cellsrdinated to allow proper organismal development. Here, using mouse embryonic stem cells (mESCs) and mouse epiblast-like cells (mEpiLCs) as model systems, we describe a robust protocol that allows comprehensive and comparative structural analysis of the glycome.

insolence 发表于 2025-3-28 20:31:00

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FATAL 发表于 2025-3-29 02:45:31

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对手 发表于 2025-3-29 06:34:30

Perry J. Samson,Jennie L. Moodyipid-based transfection systems. Herein we describe a forward transfection protocol which we routinely use to achieve upwards of 70% transfection efficiency rates in mEpiSCs. Our protocol also includes a suggested transfection timeline and details pertaining to the techniques we use to validate transfection success.

雄伟 发表于 2025-3-29 08:42:19

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动机 发表于 2025-3-29 15:27:55

W. E. Davis,A. R. Olsen,B. T. Didier process up to 96 cells from a single run with custom library preparation. The method enables detection of Transcription Start Site (TSS) at the single-cell resolution yielding a more comprehensive overview of gene regulatory elements governing in the EpiSC-like cell (EpiLC) including non-polyadenylated RNA and enhancer RNA activities.

白杨 发表于 2025-3-29 18:35:18

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暂时休息 发表于 2025-3-29 23:20:17

Volatile Organic Compounds (VOCs) Control,persed colonies, have an increased proliferation rate, and a predisposition to neural fate. EPL cells can be used to study the cell states along the pluripotency continuum, peri-implantation embryogenesis, and as a starting point for efficient neuronal differentiation.

Devastate 发表于 2025-3-30 03:14:01

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Glaci冰 发表于 2025-3-30 06:14:38

L-Proline Supplementation Drives Self-Renewing Mouse Embryonic Stem Cells to a Partially Primed Plurpersed colonies, have an increased proliferation rate, and a predisposition to neural fate. EPL cells can be used to study the cell states along the pluripotency continuum, peri-implantation embryogenesis, and as a starting point for efficient neuronal differentiation.
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查看完整版本: Titlebook: Epiblast Stem Cells; Methods and Protocol Pierre Osteil Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license