外露
发表于 2025-3-25 06:32:10
M. van Loon,P. J. H. Builtjes,A. J. SegersThe ability to generate primordial germ cell-like cells (PGCLCs) from murine embryonic stem cells (ESCs) has enabled in vitro investigation of the molecular mechanisms regulating this process without the use of a mouse model. Here we describe the procedures from the culture of ESCs to the detection of PGCLCs in the embryoid bodies (spheroids).
哑剧
发表于 2025-3-25 11:00:48
A Reproducible and Dynamic Workflow for Analysis and Annotation of scRNA-Seq DataSingle-cell RNA-sequencing (scRNA-Seq) is a widely used technology to reveal the heterogeneity and dynamics of tissues, organisms, and complex diseases. Here, a workflow is presented for preprocessing of scRNA-seq data to quantify gene abundances in individual cells followed by visualization and annotation of cells.
intrigue
发表于 2025-3-25 13:57:42
In Vitro Differentiation of Murine Embryonic Stem Cells (ESCs) into Primordial Germ Cell-like Cells The ability to generate primordial germ cell-like cells (PGCLCs) from murine embryonic stem cells (ESCs) has enabled in vitro investigation of the molecular mechanisms regulating this process without the use of a mouse model. Here we describe the procedures from the culture of ESCs to the detection of PGCLCs in the embryoid bodies (spheroids).
Ingrained
发表于 2025-3-25 19:29:06
Heinz Brauer,Yalamanchili B. G. Varmat stem cells as they share similar properties, such as their incapability to contribute to the formation of an embryo after injection into blastocyst. The epiblast stem cells (EpiSC) have delineated a novel status of pluripotency called “primed.” How to establish EpiSC from mouse embryo is described in detail in this chapter.
Notorious
发表于 2025-3-25 23:55:38
PPI-Theory for Particle Dispersion microscopy analysis. Colocalization assessment of nuclear and cytoplasmic cell regions is detailed to demonstrate nuclear and cytoplasmic localization in mEpiSCs by confocal microscopy and orthogonal colocalization assessment. Protein colocalization within mESCs, mEpiLCs, and mEpiSCs can be efficiently completed using these optimized protocols.
胎儿
发表于 2025-3-26 03:56:59
Establishment of Mouse Epiblast Stem Cellst stem cells as they share similar properties, such as their incapability to contribute to the formation of an embryo after injection into blastocyst. The epiblast stem cells (EpiSC) have delineated a novel status of pluripotency called “primed.” How to establish EpiSC from mouse embryo is described in detail in this chapter.
osculate
发表于 2025-3-26 06:21:01
3D Immunofluorescent Image Colocalization Quantification in Mouse Epiblast Stem Cells microscopy analysis. Colocalization assessment of nuclear and cytoplasmic cell regions is detailed to demonstrate nuclear and cytoplasmic localization in mEpiSCs by confocal microscopy and orthogonal colocalization assessment. Protein colocalization within mESCs, mEpiLCs, and mEpiSCs can be efficiently completed using these optimized protocols.
同步左右
发表于 2025-3-26 08:31:19
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fibroblast
发表于 2025-3-26 14:21:54
Air Quality Modeling in Complex Terrainsn proteins and chromatin. Compared to previous methods, MNase-seq and DNase-seq, ATAC-seq requires only 50,000 cells, orders of magnitude fewer cells. In addition, the ATAC-seq protocol takes one day to progress from cells to sequencing ready libraries.
歪曲道理
发表于 2025-3-26 19:46:46
Jutta Graf,Hans Schlager,Monika Krautstrunkrdinated to allow proper organismal development. Here, using mouse embryonic stem cells (mESCs) and mouse epiblast-like cells (mEpiLCs) as model systems, we describe a robust protocol that allows comprehensive and comparative structural analysis of the glycome.