先驱
发表于 2025-3-26 21:49:44
Investigation of Amphiphilic Nature of Different Forms of Acetylcholinesterase from Torpedo Marmoratre attached to collagen-like tails forming a triple helix. DS-AChE is a dimer with a S-value of 6.5S in the presence of Triton X-100 and has a subunit molecular weight of 66,000. By limited digestion of DS-AChE with proteinase K a catalytically active enzyme sedimenting at 7.5S is formed.
Simulate
发表于 2025-3-27 03:17:49
Separation of Hydrophobic Membrane Proteins by Phase Partition: Characterization by Two-Dimensional e partition. An elegant version of the latter technique was described by Bordier (1981). This consists of using a non-ionic detergent with a cloud-point somewhat higher than room temperature to partition proteins into hydrophobic and hydrophilic fractions.
faction
发表于 2025-3-27 09:16:38
http://reply.papertrans.cn/32/3132/313123/313123_33.png
Gudgeon
发表于 2025-3-27 11:11:33
http://reply.papertrans.cn/32/3132/313123/313123_34.png
自爱
发表于 2025-3-27 16:50:51
http://reply.papertrans.cn/32/3132/313123/313123_35.png
Somber
发表于 2025-3-27 21:05:33
http://reply.papertrans.cn/32/3132/313123/313123_36.png
诱导
发表于 2025-3-28 01:04:03
http://reply.papertrans.cn/32/3132/313123/313123_37.png
MULTI
发表于 2025-3-28 03:46:58
http://reply.papertrans.cn/32/3132/313123/313123_38.png
COMMA
发表于 2025-3-28 10:09:37
Selective Labeling of the ADP/ATP Translocator with Eosin-5-Maleimidecontains 4 cysteins over 297 amino acids. Masking or modification of sulfydryl groups, which are essential for the nucleotide translocation, causes the inhibition of the translocation activity. At least one sulfydryl group is maked when CAT is bound to the translocator.
使痛苦
发表于 2025-3-28 12:30:50
Site-Directed Hydrophobic Labeling of Membrane Proteins: NBD-Modification of Bacteriorhodopsinintramembraneous disposition of a protein arises from hydrophobic labeling studies. Those segments of intrinsic membrane proteins that are in close association with the hydrocarbon core of a lipid bilayer may be identified by using hydrophobic photogenerated reagents (Gitler and Bercovici 1980, Brunner and Semenza 1981, Ross et al. 1982).