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Whole-Brain Clearing and Immunolabelling in the African Killifishbiological processes. Yet, the opaqueness of fixed samples prevents the visualization of large volumes of tissue. Tissue clearing allows to match the refractive indices of biological specimens with the mounting media, allowing light penetration and imaging of millimetric depths within tissues. We p有助于 发表于 2025-3-22 11:47:54
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The Chromatin Immunoprecipitation (ChIP) Protocol for African Turquoise Killifishrofiling their genomic distribution on a genome-wide scale is of crucial significance for decoding the underlying gene regulatory networks. Chromatin immunoprecipitation followed by high-throughput DNA sequencing (ChIP-seq) is a powerful and extensively employed approach for studying in vivo proteinEmployee 发表于 2025-3-22 17:32:19
EdU Labeling of Proliferating Cells in the African Killifishcontext of development, wound healing, and regeneration. In this chapter, we present a protocol to perform intraperitoneal injection of EdU to label DNA of dividing killifish cells. After a short in vivo labeling pulse of EdU, we list detailed instructions and important considerations for tissue co不要严酷 发表于 2025-3-22 23:49:29
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