贪污
发表于 2025-3-21 19:28:33
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fetter
发表于 2025-3-21 22:49:45
Guglielmo Chiodi,Leonardo Dittaversity is generated entirely in vitro, and the protocol, involving two rounds of PCR with no gel purifications, can be accomplished easily in less than one day, followed by cloning as one would for a traditional PCR product.
收藏品
发表于 2025-3-22 03:57:02
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看法等
发表于 2025-3-22 06:49:37
Construction of Designed Protein Libraries Using Gene Assembly Mutagenesisversity is generated entirely in vitro, and the protocol, involving two rounds of PCR with no gel purifications, can be accomplished easily in less than one day, followed by cloning as one would for a traditional PCR product.
Cardioversion
发表于 2025-3-22 11:13:57
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平静生活
发表于 2025-3-22 13:53:22
Saturation Mutagenesisms, and study structure-function relationships. With saturation mutagenesis, it is possible to create a library of mutants containing all possible mutations at one or more pre-determined target positions in a gene sequence.
平静生活
发表于 2025-3-22 17:13:55
DNA Shuffling annealing, and extension by a polymerase (..). Recombination occurs when fragments from different parents anneal at a region of high sequence identity. Following this reassembly reaction, PCR amplification with primers is used to generate full-length chimeras suitable for cloning into an expression vector.
确定方向
发表于 2025-3-22 23:55:44
In Vitro DNA Recombination by Random Priminggical systems (.,.). As compared to random mutagenesis, recombination may be advantageous in combining beneficial mutations that have arisen independently and may be synergistic, while simultaneously removing deleterious mutations.
松软
发表于 2025-3-23 04:44:15
Preparation of SCRATCHY Hybrid Protein Librariese identity between the original sequences (.). Such multi-crossover hybrids can be of interest to the studies of fundamental questions of protein evolution and folding, as well as to the tailoring of enzymes for therapeutic and industrial applications.
Allodynia
发表于 2025-3-23 05:43:24
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