outset
发表于 2025-3-23 11:32:38
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许可
发表于 2025-3-23 14:48:15
1064-3745easily redesigned by similar approaches. A simple algorithm of iterative cycles of diversification and selection, evolution works at all scales, from single molecules to whole ecosystems. In the little more than a decade since the first reported applications of evolutionary design to enzyme enginee
Grating
发表于 2025-3-23 19:07:50
https://doi.org/10.1007/978-981-97-3027-8ms, and study structure-function relationships. With saturation mutagenesis, it is possible to create a library of mutants containing all possible mutations at one or more pre-determined target positions in a gene sequence.
轿车
发表于 2025-3-24 01:27:19
Gaben von Nachiketas und Savitri annealing, and extension by a polymerase (..). Recombination occurs when fragments from different parents anneal at a region of high sequence identity. Following this reassembly reaction, PCR amplification with primers is used to generate full-length chimeras suitable for cloning into an expression vector.
纯朴
发表于 2025-3-24 05:23:20
,The Existence of Augustinian ‘Metaphysics’,gical systems (.,.). As compared to random mutagenesis, recombination may be advantageous in combining beneficial mutations that have arisen independently and may be synergistic, while simultaneously removing deleterious mutations.
Frequency-Range
发表于 2025-3-24 07:52:33
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与野兽博斗者
发表于 2025-3-24 11:50:08
Generating Mutant Libraries Using Error-Prone PCRolymerase (.,.). . polymerase (.) is commonly used because of its naturally high error rate, with errors biased toward AT to GC changes. However, recent protocols include the use of a newly-developed polymerase whose biases allow for increased variation in mutation type (i.e., more GC to AT changes) (..).
SNEER
发表于 2025-3-24 16:12:23
Evolution of Microorganisms Using Mutator Plasmids.). It is well documented that mutator strains can evolve rapidly to produce offsprings with novel phenotypes. However, their application for research or as industrial production hosts has been prevented by their very limited genetic stability.
resilience
发表于 2025-3-24 20:33:31
Guglielmo Chiodi,Leonardo Dittaso-called “background” ligation products). Furthermore, ligation and transformation of the recombinant plasmids must be performed using materials and conditions that yield a sufficient number of transformants (∼10.–10.) for identifying variants exhibiting desired properties.
CRASS
发表于 2025-3-25 01:16:56
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