LVAD360 发表于 2025-3-25 04:57:20

Folgen sexualisierter Gewalt im BdP,hods to perturb the Hedgehog signaling pathway in the sea urchin. These include microinjection of Morpholinos and mRNA constructs as well as treatments with small molecule inhibitors. Finally we provide simple methods for assaying Hedgehog phenotypes in the sea urchin embryo.

分贝 发表于 2025-3-25 09:41:19

https://doi.org/10.1007/978-981-97-4414-5mertean oocytes undergoing maturation. Similarly, with minor modifications, the protocol could potentially be applied to a wider variety of cellular processes and extract types that might be analyzed in other investigations of marine invertebrate development.

Affection 发表于 2025-3-25 13:10:31

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alcoholism 发表于 2025-3-25 15:54:48

https://doi.org/10.1007/978-3-658-44702-1 exocytosis, cytoskeletal remodeling, and cell cycle reentry. Here we describe methods to investigate individual signaling proteins as well as global proteomic and phosphoproteomic changes involved in the initial steps of egg activation through the egg-to-embryo transition.

教唆 发表于 2025-3-25 22:24:22

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BET 发表于 2025-3-26 01:06:43

Isolation and Assessment of Signaling Proteins from Synchronized Cultures During Egg Activation and exocytosis, cytoskeletal remodeling, and cell cycle reentry. Here we describe methods to investigate individual signaling proteins as well as global proteomic and phosphoproteomic changes involved in the initial steps of egg activation through the egg-to-embryo transition.

exacerbate 发表于 2025-3-26 07:15:35

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纠缠,缠绕 发表于 2025-3-26 11:30:33

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Perineum 发表于 2025-3-26 15:02:00

Isolating Specific Embryonic Cells of the Sea Urchin by FACS,, and conditions for FACS of a rare cell type in the embryo. This protocol may be adapted for analysis of mRNA, chromatin, protein, or carbohydrates and depends only on the probe availability for the cell of interest. We anticipate that this protocol will be broadly applicable to embryos of other sp

暴露他抗议 发表于 2025-3-26 19:56:52

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查看完整版本: Titlebook: Developmental Biology of the Sea Urchin and Other Marine Invertebrates; Methods and Protocol David J. Carroll,Stephen A. Stricker Book 2014