Fresco 发表于 2025-3-26 22:11:29
Selection of Aptamer for ,-Methyl Mesoporphyrin IX to Develop Porphyrin Metalation DNAzymeing to the metalation of MPIX. The DNA aptamers for porphyrin molecules usually display different catalytic functions (termed deoxyribozymes or DNAzymes), which can accelerate such chemical reactions. Inspired by this, an affinity chromatography selection approach was designed for identifying a porp斗争 发表于 2025-3-27 04:57:18
PCR Methods for the Generation of Catalytic ssDNAyribozymes, but is particularly important for performing in vitro selections. While the production of single-stranded RNA is straightforward by means of in vitro transcription, the enzymatic production of single-stranded DNA (ssDNA) on a preparative scale is often difficult. Nevertheless, several me贪婪地吃 发表于 2025-3-27 08:39:42
http://reply.papertrans.cn/27/2603/260232/260232_33.png冥界三河 发表于 2025-3-27 11:25:28
http://reply.papertrans.cn/27/2603/260232/260232_34.pngmyocardium 发表于 2025-3-27 13:44:25
http://reply.papertrans.cn/27/2603/260232/260232_35.pngExtemporize 发表于 2025-3-27 18:36:36
http://reply.papertrans.cn/27/2603/260232/260232_36.pngHeretical 发表于 2025-3-28 01:06:50
Crystallization and Structural Determination of 8–17 DNAzymed significant attention. However, due to their intrinsic difficulties in crystallization and structural determination, only very limited structural information of DNAzymes is available to date. Using co-crystallization with the . Polymerase X (.PolX) protein, we have recently solved a complex struct四牛在弯曲 发表于 2025-3-28 06:10:47
http://reply.papertrans.cn/27/2603/260232/260232_38.png宣称 发表于 2025-3-28 10:04:07
http://reply.papertrans.cn/27/2603/260232/260232_39.png断断续续 发表于 2025-3-28 11:54:04
Single-Molecule Kinetic Studies of Nucleic Acids by Förster Resonance Energy Transfer structures and the challenges specific to single-molecule observation techniques, the data recorded in such experiments must be processed via multiple statistical treatments to finally yield a reliable mechanistic view of the NA dynamics. In this chapter, we propose a comprehensive protocol to anal