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发表于 2025-4-1 02:26:20
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发表于 2025-4-1 11:24:58
Filter-Binding Assays,The concentration dependence of binding yields estimates of the equilibrium dissociation constant and trivial variations allow access to kinetic and thermodynamic data. The use of this technique is illustrated here using results from our experiments with the . methionine repressor, MetJ.
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发表于 2025-4-1 16:13:23
Use of a Reporter Gene Assay in Yeast for Genetic Analysis of DNA-Protein Interactions,henotype, such as reductions or increases in the stability of the DNA–protein complex. Following identification of the relevant mutations, the mutant protein or binding site can be subjected to further analyses to confirm the expected biochemical basis of the selected phenotype. This approach has be
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发表于 2025-4-2 00:01:14
Samuel Romine,Joshua Jensen,Robert BallThe concentration dependence of binding yields estimates of the equilibrium dissociation constant and trivial variations allow access to kinetic and thermodynamic data. The use of this technique is illustrated here using results from our experiments with the . methionine repressor, MetJ.
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发表于 2025-4-2 06:03:08
R. Shoureshi,M. Wheeler,L. Brackneyhenotype, such as reductions or increases in the stability of the DNA–protein complex. Following identification of the relevant mutations, the mutant protein or binding site can be subjected to further analyses to confirm the expected biochemical basis of the selected phenotype. This approach has be
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发表于 2025-4-2 07:34:59
Book 2009Latest editionry protocols, and notes on troubleshooting and avoiding known pitfalls....Comprehensive and authoritative, .DNA-Protein Interactions: Principles and Protocols, Third Edition. serves as an ideal guide for all those exploring this dynamic, essential, and increasingly affordable area of research..