逗留 发表于 2025-3-27 00:45:56

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enlist 发表于 2025-3-27 02:41:25

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猛击 发表于 2025-3-27 05:34:58

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Hallowed 发表于 2025-3-27 13:03:52

Lecture Notes in Networks and Systemsf extracted nucleoids. Since the few DNA nicks that occur when nucleoids are isolated (.,.) are localized by the presence of 50–100 barriers to strand rotation (.,.), it is possible to recover chromosomal DNA in which most of each molecule is topologically constrained (.,.). Consequently, intracellu

许可 发表于 2025-3-27 14:11:41

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凶兆 发表于 2025-3-27 20:16:40

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Occupation 发表于 2025-3-27 22:22:34

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闲聊 发表于 2025-3-28 05:16:00

https://doi.org/10.1007/978-3-030-42726-9nzyme from . consists of two proteins, A and B (termed GyrA and GyrB), of molecular masses 97 and 90 kDa, respectively; the active enzyme is an A.B. complex. All DNA topoisomerases are able to relax negatively supercoiled DNA, but only gyrase can also catalyze the introduction of negative supercoils

amenity 发表于 2025-3-28 09:23:18

Mohamed Ihmeida,Muhammad Shahzadcommonly used biophysical methods. This is especially true for three-dimensional structure determination using X-ray crystallography or NMR. Structure–function analysis by site-directed mutagenesis requires that a large number of mutant enzymes be expressed and purified readily, so that their proper

gimmick 发表于 2025-3-28 13:01:54

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查看完整版本: Titlebook: DNA Topoisomerase Protocols; Volume I: DNA Topolo Mary-Ann Bjornsti,Neil Osheroff Book 1999 Humana Press 1999