射手座 发表于 2025-3-23 09:53:32
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Overexpression and Purification of ,DNA Topoisomerase III,hanism of topoisomerases. The ability to overexpress and purify large quantities of these molecules has led to the elucidation of the crystal structures of the amino-terminal fragment of the GyrB subunit of . DNA gyrase (.), a large fragment of yeast topoisomerase II (topo II) (.), and of the firstCirrhosis 发表于 2025-3-24 13:12:48
Overexpression and Purification of Bacterial Topoisomerase IV,ins with a high degree of amino acid similarity to . and ., respectively. A new superhelical DNA relaxation activity could be demonstrated when extracts prepared from strains overproducing the . and . gene products were mixed together. ParC and ParE were subsequently purified (.,.), and it was demonmonopoly 发表于 2025-3-24 17:47:39
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Overexpression and Purification of DNA Topoisomerase I from Yeast,g camptothecin have been the subject of intense investigation (reviewedin (.-.). Much of this effort has focused on structure/function studies of wild-type and mutant forms of the enzyme, derived from a variety of sources (.,.-.). The budding yeast . has proven particularly amenable to the genetic mlabyrinth 发表于 2025-3-25 00:14:23
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