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Establishment of the DNA Repair-Defective Mutants in DT40 Cellsding efficient gene targeting and ease of chromosome manipulation. Although the genetic approach using the RNA interference technique has become the standard method particularly in human cells, DT40 still remains a powerful tool to investigate the regulation and function of genes and proteins in a v拱墙 发表于 2025-3-22 11:21:01
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The Comet Assay: A Sensitive Genotoxicity Test for the Detection of DNA Damage and Repairs technique, a small number of cells suspended in a thin agarose gel on a microscope slide is lysed, electrophoresed, and stained with a fluorescent DNA-binding dye. Cells with increased DNA damage display increased migration of chromosomal DNA from the nucleus towards the anode, which resembles the抱负 发表于 2025-3-22 18:56:29
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Quantitative DNA Damage and Repair Measurement with the Yeast Comet Assayive effects of chemicals. The main advantage over the comet assay using cells of higher organisms is the genetic tractability and ease of cultivation of yeast. A drawback is the lower DNA content of the cells as well as the need for cell wall digestion prior to electrophoresis. Here, we describe inMisnomer 发表于 2025-3-23 03:11:31
Analysis of DNA Damage and Repair in Nuclear and Mitochondrial DNA of Animal Cells Using Quantitativ nonmammalian species such as . (nematodes), . (fruit flies), and two species of fish (. and .). Since its development in the early 1990s (Kalinowski et al., Nucleic Acids Res 20:3485–3494, 1992; Salazar and Van Houten, Mutat Res 385:139–149, 1997; Yakes and Van Houten, Proc Natl Acad Sci USA 94:514包裹 发表于 2025-3-23 07:50:11
In Vitro DNA Mismatch Repair in Human Cellstenance. This assay has been effectively used to evaluate MMR proficiency in various tumor cells and to identify the majority of the protein components required for MMR. The procedure for setting up and performing the MMR assay involves mismatch substrate preparation, cell extract preparation, and t