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Purification of MeCP2-Containing Deacetylase from ,,diate transcriptional repression. MeCP1 is a relatively uncharacterized complex that requires at least 12 symmetrical methyl-CpGs for DNA binding (.). MeCP2 is a single polypeptide containing a methyl-binding domain capable of binding a single methyl-CpG, and a transcriptional repression domain (.).我怕被刺穿 发表于 2025-3-22 10:49:31
Measuring DNA Demethylase Activity In Vitro,te between DNA that is directly demethylated, and repair processes that remove methylated cytosines in DNA and replace them with other unmethylated cytosines found in the extracts. An additional assay is the bisulfite-mapping method, which can determine the state of methylation of cytosines at a sinear-canal 发表于 2025-3-22 13:35:30
Extracting DNA Demethylase Activity from Mammalian Cells,tivity and we defined the reactants and products of the demethylation reaction. The demethylation reaction involves the hydrolytic cleavage of the bond between the methyl-carbon and the carbon at the 5 position of the cytosine ring (. and .) producing unmethylated cytosine while the methyl group isear-canal 发表于 2025-3-22 20:30:37
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