温和女人
发表于 2025-3-25 05:55:47
http://reply.papertrans.cn/25/2428/242717/242717_21.png
善辩
发表于 2025-3-25 08:11:48
Applications and Usability of Interactive TVP-dependent motility, . represents a good model system to assess enzyme activity of diguanylate cyclases and phosphodiesterases using T4P-dependent motility as a readout. Here, we describe the assay, which allows correlating diguanylate cyclase and phosphodiesterase activity with T4P-dependent motility in ..
labyrinth
发表于 2025-3-25 12:36:02
http://reply.papertrans.cn/25/2428/242717/242717_23.png
排他
发表于 2025-3-25 18:27:32
Detection of Cyclic Dinucleotides by STING IRF3 (Interferon regulatory factor 3) for type I interferon production. Here, we describe some experimental procedures such as Isothermal Titration Calorimetry and luciferase reporter assays to study the CDNs binding and activity by STING proteins.
NAUT
发表于 2025-3-25 20:13:15
Spectrophotometric and Mass Spectroscopic Methods for the Quantification and Kinetic Evaluation of Imetry (UPLC-MS/MS). These methods can be leveraged for a number of experimental applications including the evaluation of enzyme activity for the in vitro synthesis of c-di-GMP, examination of how molecular signals impact these activities, identifying the catalytic properties of hybrid DGC-PDE proteins, and the development of DGC inhibitors.
Conduit
发表于 2025-3-26 00:27:50
Type IV Pili-Dependent Motility as a Tool to Determine the Activity of c-di-GMP Modulating Enzymes iP-dependent motility, . represents a good model system to assess enzyme activity of diguanylate cyclases and phosphodiesterases using T4P-dependent motility as a readout. Here, we describe the assay, which allows correlating diguanylate cyclase and phosphodiesterase activity with T4P-dependent motility in ..
健忘症
发表于 2025-3-26 05:59:01
http://reply.papertrans.cn/25/2428/242717/242717_27.png
提炼
发表于 2025-3-26 10:16:06
Zhuohua Liu,Wei Zhang,Chuankun Wuaphy (RP-HPLC) analysis has become a commonly used approach to accomplish these goals. The following describes the extraction and HPLC-based detection and quantification of c-di-GMP from . samples, a procedure that is amenable to modifications for the analysis of c-di-GMP in other bacterial species.
废墟
发表于 2025-3-26 13:10:05
Low-Data Complexity Attacks on Camelliantrations have been developed, they are time intensive, expensive, low-throughput, and incapable of directly monitoring dynamic changes in vivo. In this protocol, we provide a .-specific detailed methodology to assay the intracellular levels of cyclic di-GMP using biological reporters.
athlete’s-foot
发表于 2025-3-26 20:19:56
http://reply.papertrans.cn/25/2428/242717/242717_30.png