错
发表于 2025-3-23 13:30:23
Gauging and Visualizing c-di-GMP Levels in , Using Fluorescence-Based Biosensorsdescribe protocols for the use of these biosensors in gauging and visualizing cellular c-di-GMP levels of . both in in vitro setups such as continuous-culture flow-cell biofilms, and in in vivo settings such as a murine corneal infection model.
一小块
发表于 2025-3-23 15:14:29
High-Performance Liquid Chromatography (HPLC)-Based Detection and Quantitation of Cellular c-di-GMPaphy (RP-HPLC) analysis has become a commonly used approach to accomplish these goals. The following describes the extraction and HPLC-based detection and quantification of c-di-GMP from . samples, a procedure that is amenable to modifications for the analysis of c-di-GMP in other bacterial species.
deadlock
发表于 2025-3-23 18:34:13
Cyclic di-GMP-Responsive Transcriptional Reporter Bioassays in ,ntrations have been developed, they are time intensive, expensive, low-throughput, and incapable of directly monitoring dynamic changes in vivo. In this protocol, we provide a .-specific detailed methodology to assay the intracellular levels of cyclic di-GMP using biological reporters.
kidney
发表于 2025-3-24 00:15:13
http://reply.papertrans.cn/25/2428/242717/242717_14.png
得意人
发表于 2025-3-24 04:27:22
Yufeng Lin,Biplob Ray,Dennis Jarvis,Jia WangG) and 5′-guanosine monophosphate (5′-GMP), as well as cyclic guanosine monophosphate (3′,5′-cGMP) and 3′,3′ c-di-GMP analogues (2′,3′-c-di-GMP and 2′,2′-c-di-GMP) can be simultaneously determined by this method.
callous
发表于 2025-3-24 10:20:23
https://doi.org/10.1007/978-981-10-5421-1ng for diguanylate cyclase activity, (2) analyzing c-di-GMP levels under anaerobic conditions, and (3) monitoring cell population dynamics of c-di-GMP levels upon environmental changes. These methods showcase RNA-based fluorescent biosensors as versatile tools for studying c-di-GMP signaling in bacteria.
使服水土
发表于 2025-3-24 14:17:55
http://reply.papertrans.cn/25/2428/242717/242717_17.png
整顿
发表于 2025-3-24 17:34:17
Applications and Usability of Interactive TV and biofilm phenotypes in . and chemotactic behavior in the alphaproteobacterium .. We expect these light-activated enzymes to be also useful in regulating c-di-GMP-dependent processes occurring at the fast timescale, for spatial control of bacterial populations, as well as for analyzing c-di-GMP-dependent phenomena at the single-cell level.
wall-stress
发表于 2025-3-24 20:29:44
http://reply.papertrans.cn/25/2428/242717/242717_19.png
crockery
发表于 2025-3-25 02:50:17
Lynn Margaret Batten,Mohammed Khalil Amain IRF3 (Interferon regulatory factor 3) for type I interferon production. Here, we describe some experimental procedures such as Isothermal Titration Calorimetry and luciferase reporter assays to study the CDNs binding and activity by STING proteins.