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Flat Corrugated and Bull’s-Eye AntennasSomatic mutations in the p53 gene is the most common genetic abnormality so far described in human cancer (.). Patients with germ-line p53 mutation’s are part of the Li-Fraumeni syndrome. These patients have an increased risk of developing adrenocortical, breast, gastrointestinal tract, and lung car
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发表于 2025-3-30 15:51:13
https://doi.org/10.1007/978-1-4899-3434-5ll culture to produce metaphase spreads. Fluorescence . hybridization (FISH) is a powerful method for the analysis of genetic change in solid tumors (.,.–.). In particular, it allows the visualization of the genetic makeup of individual cells within their histological context (.). In general, FISH u
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1543-1894 toxic Drug Resistance Mechanisms is to provide pro- cols that are appropriate for examining the mechanisms of cellular resistance to anticancer cytotoxics in hu978-1-61737-093-9978-1-59259-687-4Series ISSN 1543-1894 Series E-ISSN 1940-6037
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发表于 2025-3-30 21:28:46
Cell Sensitivity Assays, of individual cells to form colonies. However, cytotoxicity assays can also measure the ability of cells to proliferate if the cells are allowed a period of growth following drug exposure. This recovery time is comparable to the time taken for formation of colonies in a clonogenic assay.
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5-Fluorouracil Metabolizing Enzymes,hydropyrimidine dehydrogenase (DPD; EC 1.3.1.2). Following a bolus injection of 5-FU, 80% is degraded via DPD after 24 h after administration (.). Studies of .F nuclear magnetic resonance (NMR) spectroscopy in mice bearing colon tumors found catabolites made up 51% of labeled drug in the tumor, comp
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Measuring DNA Adducts by Immunoassay (ELISA),Finally, a knowledge of the DNA adducts levels formed in patients during therapy permits direct assessment of the clinical relevance of the levels of DNA modification employed in in vitro experiments. This could be important because the nature and significance of the various types of cellular respon
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发表于 2025-3-31 21:12:04
Analysis of the p53 Status of Tumors,Somatic mutations in the p53 gene is the most common genetic abnormality so far described in human cancer (.). Patients with germ-line p53 mutation’s are part of the Li-Fraumeni syndrome. These patients have an increased risk of developing adrenocortical, breast, gastrointestinal tract, and lung car
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