洁净
发表于 2025-3-28 16:18:25
Analysis of Calcium-Sensing Receptor Signaling Using Dual Luciferase Assaysmoter driving the expression of a second luciferase, namely, . luciferase, whose activity can be quantified within the same sample. Here, a protocol for the analysis of CaSR signaling using dual luciferase assays in HEK293 cells is provided. The assays can, for example, be used to investigate functi
Adrenal-Glands
发表于 2025-3-28 22:03:11
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GROVE
发表于 2025-3-29 01:32:14
Monitoring ER Ca2+ by Luminescence with Low Affinity GFP-Aequorin Protein (GAP)owing long-term (longer than 1 h) ER Ca. measurements. GAP1 targeted to the ER allows monitoring of resting . and Ca. dynamics in intact cells stimulated with IP.-produced agonists. In addition, GAP1 can record Ca. mobilization in permeabilized cells challenged with IP.. We also provide a detai
judiciousness
发表于 2025-3-29 04:34:10
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contrast-medium
发表于 2025-3-29 09:33:00
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defibrillator
发表于 2025-3-29 12:04:49
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冲突
发表于 2025-3-29 17:07:38
https://doi.org/10.1007/978-94-015-8348-0a mammalian expression construct comprising the full-length human CaSR with an N-terminal bungarotoxin minimal-binding site that can be labeled with commercially available fluorescent ligands to measure endocytosis, and a super-ecliptic pHluorin (SEP) to measure total cell surface expression and exo
吵闹
发表于 2025-3-29 20:28:02
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Hiatal-Hernia
发表于 2025-3-30 03:28:09
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GUEER
发表于 2025-3-30 05:24:04
https://doi.org/10.1007/978-1-0716-4164-4Calcium monitoring; Cellular assays; Microscopy; Luminescent reporters; Data analysis