indemnify
发表于 2025-3-28 15:05:02
Detection of Cohesin SUMOylation In Vivoe usually low and de-conjugation during sample preparation often occurs. In this chapter we describe a method that can be adapted to different model organisms, and substrates to detect SUMOylation. We focus on cohesin and show that SUMOylation indeed occurs in most of the subunits of budding yeast cohesin.
Keshan-disease
发表于 2025-3-28 19:34:24
The Use of Laser Microirradiation to Investigate the Roles of Cohesins in DNA Repairhigher eukaryotes, in DNA repair in human cells. In this review, we briefly describe what we know about cohesin function and regulation in response to DNA damage, and discuss the optimized laser microirradiation conditions used to analyze cohesin responses to DNA damage in vivo.
PACT
发表于 2025-3-29 00:01:26
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COKE
发表于 2025-3-29 05:47:10
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intelligible
发表于 2025-3-29 10:57:53
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Hearten
发表于 2025-3-29 12:29:24
Using Fluorescent Reporters in Conjunction with Cytometry and Statistics to Assess Nuclear Accumulatproteins using cytometry and biostatistics. This assay was developed for use with GFP reporters for ribosome biogenesis in budding yeast but could be adapted for use with any GFP reporter that accumulates into the nucleus under adverse conditions.
languor
发表于 2025-3-29 16:23:11
https://doi.org/10.1007/978-94-007-1324-6d chromosomes using egg extracts, along with cytological and biochemical assays for addressing the molecular mechanisms of sister chromatid cohesion. A powerful approach involving immunodepletion of cohesin and its regulators is also included.
Sedative
发表于 2025-3-29 20:08:30
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scotoma
发表于 2025-3-30 00:33:42
Laura Landi,Cinzia Tamburini,Aldo Romaniohesin is actually loaded onto chromatin. Further, Nipbl seems to have also additional roles, for instance as transcription factor..This chapter summarizes our current knowledge on kollerin function and the recent studies on the genomic localization of Scc2, highlighting and critically discussing controversial data.
模仿
发表于 2025-3-30 06:17:09
Ruzena Bajcsy,Eric Krotkov,Max Mintzscently labeled positions on the same chromosome. In combination with an automated computational analysis pipeline, this assay enables the study of various candidate proteins for their roles in regulating genome topology during cell divisions.