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, Genome Editing Using , Alleles,d subsequent isolation of double-crossover excision events using replication-defective plasmids that carry a counterselection marker. Use of a . (cytosine deaminase) or . (orotate phosphoribosyltransferase) as counter selection markers appears equally effective, but there is considerable merit in usOccipital-Lobe 发表于 2025-3-22 06:39:17
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, Adhesins,ence and on the other on the host. The pathogenesis of . infection could be seen as a three-step process that takes place after disruption of the digestive microbiota by antibiotics: (1) contamination by and germination of spores; (2) multiplication of vegetative cells in the colonic niche using col清洗 发表于 2025-3-22 19:18:04
Intestinal Epithelial Cell Response to , Flagella,and animals, produces toxins that are known to contribute directly to damage of the gut. It is known that bacterial flagella are involved in intestinal lesions through the inflammatory host response. The . flagellin recognizes TLR5 and consequently activates the NF-κB and the MAPK signaling pathways召集 发表于 2025-3-22 21:57:10
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Inducing and Quantifying , Spore Formation,aerobe to form metabolically dormant spores that can resist antibiotic treatment, survive exit from the mammalian host, and transmit . infections. In this chapter, we describe a method for inducing . sporulation in vitro. This method can be used to study sporulation and maximize spore purification y阴谋小团体 发表于 2025-3-23 08:22:47
Characterization of Functional Prophages in ,,ated within the chromosome of their host, or as episomal DNAs. Since prophages are ubiquitous throughout the bacterial world, there has been a sustained interest in trying to understand their contribution to the biology of their host. . is no exception to that rule and with the recent release of hun