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Jolanta Chmielik,Natalia Grabarbecome known as & quote;real-time& quote; PCR. Absolute quantification of initial template copy number can be obtained, although quantification relative to a control sample or second sequence is often adequate. Melting analysis following PCR monitors duplex hybridization as the temperature is change
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Gründe für die wahrscheinliche Entwicklungtwo analytes differ by 1 Da can be distinguished) and provides high sensitivity (less than 1 fmole of a DNA oligonucleotide can be detected), allowing DNA quantifications with single base specificity and single DNA molecule sensitivity (coupled with polymerase chain reaction ). To quantify a DN
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