hauteur
发表于 2025-3-23 13:12:22
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柱廊
发表于 2025-3-23 15:28:42
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文件夹
发表于 2025-3-23 19:26:27
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松鸡
发表于 2025-3-23 23:59:33
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开玩笑
发表于 2025-3-24 06:03:29
Quan-Lin Li,Rui-Na Fan,Zhi-Yong Qianciferase-based techniques require expensive substrates and are typically performed in endpoint format. Here, we describe a versatile reporter gene assay to observe nuclear receptor activity with fluorescent proteins as reporters. This setting is highly cost-efficient and enables observation of nucle
impaction
发表于 2025-3-24 09:39:20
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EXUDE
发表于 2025-3-24 11:47:21
Lecture Notes in Computer Sciencesure a compound’s cellular target engagement and permeability. HiBiT CETSA method is quantitative and has higher throughput compared to the traditional Western-based CETSA. Here, we describe the protocol for a HiBiT CETSA, which utilizes a HiBiT tag derived from the NanoLuciferase (NanoLuc) that upo
女上瘾
发表于 2025-3-24 15:49:32
Jian Zhang,Zhiqiang Zhou,Tony T. Lee,Tong Yety and neuronal function. Dysregulation of STEP is linked to the pathophysiology of Alzheimer’s disease and other neuropsychiatric disorders. Experimental results from neurological deficit disease models suggest that the modulation of STEP could be beneficial in a number of these disorders. This pro
insipid
发表于 2025-3-24 20:32:53
https://doi.org/10.1007/978-3-319-69431-3a proteome-wide level. To achieve this, native lysates are first incubated with a compound, followed by a short incubation with a nonspecific protease. Binding of a compound can change accessibility at the binding site or induce other structural changes in the target. This leads to treatment-specifi
山羊
发表于 2025-3-25 01:11:02
Quantum Fluctuations in Linear Systems,c non-covalent inhibitors, understanding target engagement and selectivity is essential for determining optimal dosing and limiting potential on- or off-target toxicity. Here, we present a complementary activity-based protein profiling (ABPP) strategy for unbiased proteome-wide profiling of cysteine