Aesthete
发表于 2025-3-25 06:39:41
Dependency Properties and Routed Events, phenomena, as they allow modulation of enzymatic activities and temporal control of protein action. Here, we describe the methodology to conduct large-scale screens for chemical compounds that induce a desired phenotype in the roundworm . (.) using 96- or 384-well microtiter plates.
移动
发表于 2025-3-25 10:07:25
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fixed-joint
发表于 2025-3-25 14:32:21
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MARS
发表于 2025-3-25 17:29:06
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滴注
发表于 2025-3-25 21:45:33
https://doi.org/10.1007/978-1-4302-1603-2ssay-guided isolation is a rapid and validated method for isolation of compounds with bioactivities. This chapter describes bioassay-guided separation and purification of compounds from the crude extracts of TCMs. Two approaches including size-exclusion chromatography (SEC) and high performance liqu
transient-pain
发表于 2025-3-26 02:39:28
Dependency Properties and Routed Events,fragment hits have lower molecular weight (<300–350 Da) and lower initial potency but higher ligand efficiency when compared to those from high-throughput screening. NMR spectroscopy has been widely used for FBDD since it identifies and localizes the binding site of weakly interacting hits on the ta
撕裂皮肉
发表于 2025-3-26 07:34:28
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光亮
发表于 2025-3-26 12:29:53
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ALERT
发表于 2025-3-26 14:44:29
Whole-Organism Screening for Modulators of Fasting Metabolism Using Transgenic Zebrafishnging using traditional high-throughput approaches in vitro. Here, we describe a method that rapidly identifies small molecules with an impact on organismal energy balance in vivo. Specifically, we developed a whole-organism screen for modulators of fasting metabolism using transgenic bioluminescence-reporter zebrafish for the gluconeogenic gene .
reserve
发表于 2025-3-26 19:51:48
Bioassay-Guided Identification of Bioactive Molecules from Traditional Chinese Medicinesssay-guided isolation is a rapid and validated method for isolation of compounds with bioactivities. This chapter describes bioassay-guided separation and purification of compounds from the crude extracts of TCMs. Two approaches including size-exclusion chromatography (SEC) and high performance liquid chromatography (HPLC) are described in detail.