Phagocytes
发表于 2025-3-25 05:02:53
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南极
发表于 2025-3-25 09:53:02
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媒介
发表于 2025-3-25 11:51:35
,PROMIS: Co-fractionation Mass Spectrometry for Analysis of Protein–Metabolite Interactions,—but a comprehensive overview of protein–metabolite interactions (PMIs) is missing. Recently we devised a non-targeted method for detecting PMIs using size-exclusion chromatography followed by proteomic and metabolomic analysis: PROMIS. Under test this method was able to identify known PMIs such as enzyme–cofactor complexes as well as novel ones.
帽子
发表于 2025-3-25 16:03:29
Solvent-Induced Protein Precipitation for Drug Target Discovery,nce to solvent-induced precipitation. This chapter describes the protocol for identifying drug–target protein interactions by performing unbiased SIP with total cell lysate using a mass spectrometry-based proteomic strategy.
Flatus
发表于 2025-3-25 20:39:02
Databases and Tools to Investigate Protein-Metabolite Interactions, our knowledge on PMIs, with particular emphasis on platforms and databases to explore the highly heterogenous nature of characterized PMIs, which is likely to be an essential resource on the development of new computational approaches to predict and validate interactions based on large-scale PMI screenings.
大范围流行
发表于 2025-3-26 03:47:33
Book 2023erent organisms, in order to shed light on these important regulatory interactions. Experimental and computational strategies to characterize protein-metabolite interactions are discussed, and recent advances in enabling technologies are featured as well. Written for the highly successful .Methods i
gerrymander
发表于 2025-3-26 06:32:06
1064-3745expertsThis thorough volume explores protocols of proteome- and metabolome-wide strategies for the identification of protein-small molecule complexes in different organisms, in order to shed light on these important regulatory interactions. Experimental and computational strategies to characterize
6Applepolish
发表于 2025-3-26 11:34:15
https://doi.org/10.1007/978-3-319-66869-7stablishing their biological relevance. Here, we describe a protocol that allows the identification of multiple protein partners for one ligand. This protocol describes a straightforward methodology that can be adapted to a wide variety of organisms.
Flirtatious
发表于 2025-3-26 12:52:10
Data Science Meets Optimizationffinity purification (AP) followed by liquid chromatography–mass spectrometry (LC–MS). Aside from its speed, simplicity, and adaptability to a variety of biological systems, its main strength lies in the parallel identification, in a near-physiological environment, of a given protein’s protein and small-molecule partners.
Headstrong
发表于 2025-3-26 17:22:37
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