cornucopia
发表于 2025-3-30 12:13:42
A Brief History of Industrial R&D,ermediates are used and generated in cell-free expression systems, something that is to date not well understood. Here, we present a detailed metabolomics protocol for characterization of the small molecules in cell-free systems. We specifically focus on the analysis of . lysate.based cell-free syst
Leaven
发表于 2025-3-30 13:05:36
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commune
发表于 2025-3-30 18:45:43
Stephen J. Mezias,Mary Ann Glynntituting multicellular behaviors with synthetic cell-mimics is still a challenge because it requires efficient communication between individual compartments in large populations. This chapter presents a microfluidic method to produce large quantities of cell-mimics with highly porous, stable, and ch
放大
发表于 2025-3-30 20:52:34
Stephen W. Mezias,Elizabeth Boyleral and synthetic components. The integration of membrane proteins into these synthetic membranes is an important step towards creating biomembrane systems for uses such as artificial cellular systems, biosensors, and drug delivery vehicles. Here, we outline a technique to create hybrid membranes co
Engulf
发表于 2025-3-31 02:14:49
Elizabeth Boyle,Stephen J. Meziasional folding of these hydrophobic proteins. Equally, in artificial cell studies, controllable methods are desirable for in situ synthesis of membrane proteins that then direct reactions in the synthetic cell membrane. Here we describe a method that exploits cell-free expression systems and tunable
GRACE
发表于 2025-3-31 05:51:10
High-Throughput Experimentation Using Cell-Free Protein Synthesis Systemsare unfit to handle many cell-free reactions. Here, we describe a microfluidic method that can generate hundreds of unique submicroliter scale reactions. The method is coupled with a high yield cell-free system that can be applied for broad protein screening assays.
Erythropoietin
发表于 2025-3-31 09:25:29
Cell-Free Gene Expression978-1-0716-1998-8Series ISSN 1064-3745 Series E-ISSN 1940-6029