expansive
发表于 2025-3-25 06:15:48
https://doi.org/10.1057/9781137571250esting various combinations of tRNAs and their modifications can be evaluated in the developed system. In this chapter, we describe how to prepare this minimal system. Methods for preparing the transcribed tRNAs, their modifications, and the protein production using the set of prepared tRNAs are shown.
Robust
发表于 2025-3-25 11:10:33
A ,-Based Cell-Free Protein Synthesis System for High-Level Protein Expressiond factors to CFPS reactions. By doing this, the protein yield of EGFP was significantly improved up to approximately 400 μg/mL. In this chapter, we mainly describe the preparation of . cell extracts, expression and purification of nine translation related factors, and optimization of the .-based CFPS system for enhanced protein expression.
Endearing
发表于 2025-3-25 14:35:04
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peritonitis
发表于 2025-3-25 19:19:20
Efficient and Precise Protein Synthesis in a Cell-Free System Using a Set of In Vitro Transcribed tResting various combinations of tRNAs and their modifications can be evaluated in the developed system. In this chapter, we describe how to prepare this minimal system. Methods for preparing the transcribed tRNAs, their modifications, and the protein production using the set of prepared tRNAs are shown.
Nebulizer
发表于 2025-3-25 20:15:42
https://doi.org/10.1057/9781137550644xtract preparation workflow using CFAI media. We also provide a detailed protocol for the alternative, 2x YPTG media-based preparation process, as it represents a useful benchmark within the cell-free community.
Perceive
发表于 2025-3-26 00:27:01
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Restenosis
发表于 2025-3-26 06:54:10
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Malaise
发表于 2025-3-26 09:26:01
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百科全书
发表于 2025-3-26 16:11:31
Stephen J. Mezias,Mary Ann Glynnemically modifiable polymer membranes that can be programmed on demand with nucleus-like DNA-hydrogel compartments for gene expression. We describe expression of genes encoded in the hydrogel compartment and communication between neighboring cell-mimics through diffusive protein signals.
Conflagration
发表于 2025-3-26 17:07:57
Cell-Free Gene Expression from DNA Brushesrtificial cells with steady-state dynamics of protein synthesis. Here, we provide the basic procedure for surface patterning, DNA immobilization, capture of protein products on antibody traps and fluorescent imaging. The method of DNA brush surface patterning enables simple parallelization of cell-f