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K. -H. Hoffmann,E. Jörn,E. Schäfer,H. Weberculate dose-response. The methods would be useful to researchers in preclinical and translational research planning to move away from simplistic monolayer studies and explore 3D spheroid screens for drug safety and efficacy without substantial investment in money or time.Metamorphosis 发表于 2025-3-22 03:35:55
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https://doi.org/10.1007/BFb0077985 and resazurin. They are applicable for adherent or suspended cell lines, easy to perform, and comparably economical. Detailed protocols and notes for easier handling and avoiding pitfalls are enclosed to each assay.ORBIT 发表于 2025-3-22 13:43:15
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Assaying Mitochondrial Respiration as an Indicator of Cellular Metabolism and Fitness,to make the inner mitochondrial membrane permeable for protons and allow maximum electron flux through the electron transport chain, and rotenone and antimycin A—to inhibit complexes I and III, respectively. This chapter describes the protocol of OCR assessment in the culture of primary myotubes obtained upon satellite cell fusion.烧瓶 发表于 2025-3-23 05:13:41
Book 2017minescence-based strategies, computational, and label-free methodologies for assaying cellular viability by means of e.g. viscoelastic properties, impedance and multiphoton microscopy. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to thTriglyceride 发表于 2025-3-23 06:52:25
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