乐章
发表于 2025-3-28 16:39:30
https://doi.org/10.1007/978-3-319-43249-6ering enzymes involved in MIA biosynthesis. Here, we discuss the latest progress in understanding the roles of oxidative enzymes in MIA metabolism and describe a bioinformatic and biochemical pipeline to identify, characterize, and make use of these plant biocatalysts.
flourish
发表于 2025-3-28 21:33:33
William J. McGuire,Carina J. Fearnleyl expression as the pathway genes, and (3) isolation of homologs of TFs known to regulate structurally similar or diverse specialized metabolites in different plant species. TFs regulating TIA pathway have been isolated using either an individual or a combination of the three approaches. Here we des
altruism
发表于 2025-3-29 01:24:19
Book 2022 reproducible protocols.. Authoritative and cutting-edge, .Catharanthus roseus: Methods and Protocols .aims to be a guidebook to all researchers working at characterizing alkaloid biosynthesis and more broadly specialized metabolisms.
sinoatrial-node
发表于 2025-3-29 06:16:59
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认为
发表于 2025-3-29 09:21:45
https://doi.org/10.1007/978-3-319-44144-3omoter transactivation studies or transgene overexpression paired with downstream analyses like quantitative PCR or metabolite analysis. Our protocol takes about 16 days from sowing seeds to obtaining the results of the experiment.
dainty
发表于 2025-3-29 14:27:48
https://doi.org/10.1007/978-3-319-44144-3st, easy to perform, and results in high amount of transient expression in .. This protocol is expected to serve as valuable tool to enhance the in planta characterization of gene functions or even transiently knock-in novel enzymatic activities.
小争吵
发表于 2025-3-29 15:39:55
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Hemiparesis
发表于 2025-3-29 20:31:40
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INERT
发表于 2025-3-30 03:33:58
A Rapid and Efficient Vacuum-Based Agroinfiltration Protocol for Transient Gene Overexpression in Lst, easy to perform, and results in high amount of transient expression in .. This protocol is expected to serve as valuable tool to enhance the in planta characterization of gene functions or even transiently knock-in novel enzymatic activities.
共同给与
发表于 2025-3-30 07:01:32
1064-3745 ation advice from the experts.This volume provides updated technical approaches that have been developed to characterize monoterpene indole alkaloid metabolism in .C. roseus. from metabolite/gene product localization, alkaloid chemical synthesis, candidate gene prediction, transcription factor chara