defuse 发表于 2025-3-25 04:46:28
https://doi.org/10.1007/978-1-4020-6660-3rgical limitations. The analysis of these tissues would be assisted if contaminating serum albumin could be reduced. This chapter outlines a protocol for the effective reduction of serum albumin levels from human myocardium extracts enriched for soluble cytoplasmic proteins.Cubicle 发表于 2025-3-25 09:42:14
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Two-Dimensional Polyacrylamide Gel Electrophoresis for Cardiovascular Proteomicsinely can be applied to parallel quantitative expression profiling of large sets of complex protein mixtures, such as those represented by cardiovascular cell and tissue lysates. This chapter details a procedure for large-format 2DE, and its variations, that has been successfully applied in cardiovascular proteomic research.Mechanics 发表于 2025-3-26 00:37:48
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Jan C. A. Boeyens,Demetrius C. Levendisllowing the analysis of proteins in their physiologic/ intracellular context, has become the most commonly used preparative/enrichment method. This chapter introduces state-of-the-art subcellular fractionation protocols and briefly discuss their suitability, advantages, and limitations.