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https://doi.org/10.1007/978-3-642-19294-4rates that cancer stem cells can survive and generate colony growth in an anchorage-independent culture model. The 0.005% crystal violet solution is used in this assay to visualize the generated colonies.用不完 发表于 2025-3-22 02:01:49
https://doi.org/10.1007/978-4-431-76428-1ion of the explain immunohistochemical methods used in the characterization of CSCs. Furthermore, focus on the most common troubleshooting in CSCs IHC is provided, especially the pitfalls of the CSCs markers IHC on tissue microarrays.暂时休息 发表于 2025-3-22 05:20:34
International Year of Planet Earthand other cell types that prevail in the tumor milieu. In this chapter, we have described the methodology to develop in vitro 3D tumor models that incorporate these microenvironmental characteristics and design experiments that generate endpoints for understanding radioresistance in cancer stem cells.achlorhydria 发表于 2025-3-22 12:24:42
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Book 2018tion of cancer stem cells. These include surface marker expression, side population, spheres formation, ALDH activity, and numerous others. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materapiary 发表于 2025-3-22 18:39:53
In Vitro Tumorigenic Assay: The Tumor Spheres Assay,-free medium supplemented with growth factors. Tumor spheres assays have been used in cancer research as an intermediate in vitro cell culture model to be explored before performing more laborious in vivo tumor xenograft assays.丧失 发表于 2025-3-22 22:27:55
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Immunohistochemistry for Cancer Stem Cells Detection: Principles and Methods,ion of the explain immunohistochemical methods used in the characterization of CSCs. Furthermore, focus on the most common troubleshooting in CSCs IHC is provided, especially the pitfalls of the CSCs markers IHC on tissue microarrays.staging 发表于 2025-3-23 09:20:41
Book 2018ials and reagents, step-by-step, readily reproducible protocols, and expert tips and troubleshooting advice to avoid potential pitfalls. .Comprehensive and practical, .Cancer Stem Cells: Methods and Protocols. provides researchers with the techniques they will need in order to pursue vital research into this complex field of study. .