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TUNEL and Immunofluorescence Double-Labeling Assay for Apoptotic Cells with Specific Antigen(s)ssay, and antigens of interest, using immunofluorescence, is described. The assay has been used successfully on fixed, cultured cells and formalin-fixed paraffin-embedded tissue sections. This . detection system can be used to correlate apoptosis with specific antigen expression to identify factors无情 发表于 2025-3-22 22:49:57
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Protein Tyrosine Kinase and Phosphatase Expression Profiling in Human Cancerse of the large number of tyrosine kinase/phosphatase genes in such gene family, it is essential to use an efficient and simple approach to obtain comprehensive protein tyrosine kinase and protein tyrosine phosphatase expression profiles. Knowledge of such an overall expression pattern of tyrosine ki烦人 发表于 2025-3-23 06:34:22
Association of Nonreceptor Tyrosine Kinase ,-Yes with Tight Junction Protein Occludin by Coimmunoprean immunoprecipitation procedure: 1) lysis of the cells to make the antigen of interest available; 2) formation of the antibody-antigen complex by adding specific antibody; 3) separation and detection of the immune complex by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and i