delusion 发表于 2025-3-23 13:09:13
Manipulating Expression of Endogenous Oncogenic Proteins Using an Antisense Oligonucleotide Approachoteins. Using this method, we have acquired additional evidence that protein kinase C-epsilon functions as an oncogenic protein in the progression of recurrent human prostate cancer. This chapter describes the use of antisense ODN to directly target cellular protein kinase C-epsilon as a potential cANA 发表于 2025-3-23 17:31:00
Measurements of Phospholipases A2, C, and D (PLA2, PLC, and PLD)to mitosis. Phospholipases A., C, and D (PLA., PLC, and PLD) are involved in regulatory events of intracellular mitogen signaling pathways. We describe here three methods for comprehensively assaying those phospholipases: 1) in vitro microassays, in which a radiolabeled substrate is exogenously addehieroglyphic 发表于 2025-3-23 20:04:43
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Introduction to the Study of Stress,it allows the measurement of, in parallel, the activities of three phospholipases utilizing aliquots derived from . biological sample. The approach for immunoprecipitation described in this chapter can be extrapolated to the study of a large array of enzyme isoforms. Finally, the intact-cell assaysSpinal-Fusion 发表于 2025-3-24 20:36:45
https://doi.org/10.1007/978-3-031-00864-1ed by adding to EMSA an antibody specific for that protein, which will result in supershift complexes on the gel. The presence of the proteins in question can be further verified by standard Western analysis of the nuclear proteins.Odyssey 发表于 2025-3-25 02:00:11
Association of Nonreceptor Tyrosine Kinase ,-Yes with Tight Junction Protein Occludin by Coimmunopreterest is tyrosine phosphorylated, and this tyrosine phosphorylation is under investigation, each step has to be handled with extra care to ensure success of the procedure. The buffer used to lyse the cells depends on the nature of the antigen, with more stringent conditions required for integral me