笔记 发表于 2025-3-21 17:31:02
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https://doi.org/10.1007/978-1-0716-2376-3Biobanking; chemotaxis assay; Flow-cytometry; CRISPR/Cas; Immunofluorescencepanorama 发表于 2025-3-22 02:38:19
978-1-0716-2378-7The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Businesglisten 发表于 2025-3-22 05:52:02
Sherri L. ChristianIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the expertsAncestor 发表于 2025-3-22 09:14:48
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/221058.jpg走调 发表于 2025-3-22 16:07:38
Cancer Cell Biology978-1-0716-2376-3Series ISSN 1064-3745 Series E-ISSN 1940-6029走调 发表于 2025-3-22 18:59:18
Nicholas L. Zalewski,Sean J. Pittockancer. Here we describe a method using tandem mass tag (TMT) technology to multiplex up to 18 samples in a single analysis, paving the way for the analysis of large cohorts of tumors, cell lines, and perturbations thereof. The procedure we describe will result in samples ready for in-depth LC-MS/MS analysis in 3–4 days.Nausea 发表于 2025-3-22 21:30:15
Robert L. Sainburg,Pratik K. Muthas and maintaining records on usage and confirmation of identity. Cross-contamination of cancer cell lines has been well documented by the ATCC and must be avoided at all costs. In addition, contamination by . sp. is a well-known issue, which, if left unchecked, can affect all data collected as thisneedle 发表于 2025-3-23 03:19:52
http://reply.papertrans.cn/23/2211/221058/221058_9.pngcultivated 发表于 2025-3-23 07:57:53
Volker Dietz,Tobias Nef,William Zev Rymerired to lift the cells from the cell culture vessel for harvesting or subculturing. Many cancer cell lines are adherent, rendering adherent cell culture a critical experimental method in the fields of cell biology, biochemistry, and cancer research. In this chapter, we outline the protocols for cult