NAV 发表于 2025-3-23 10:23:53
https://doi.org/10.1007/978-3-662-32527-8 the application of a calcium ionophore, such as A23187or ionomycin, to provoke an intracellular situation similar to the digital approach discussed in chapter 2 - Direct evidence - the digital approach. An even more elegant approach is to induce a transient disturbance in cardiomyocytes, the applic额外的事 发表于 2025-3-23 17:29:26
http://reply.papertrans.cn/23/2209/220811/220811_12.png临时抱佛脚 发表于 2025-3-23 22:01:46
http://reply.papertrans.cn/23/2209/220811/220811_13.png丰满有漂亮 发表于 2025-3-24 00:11:09
Grundsatz der Entgeltgleichheit im AGG,alcium signalling has not been written. Although certain effects of calcium in red blood cells have been known for more than 50 years, the means by which calcium enters the red blood cells still has not been completely elucidated. However, what has been clear for quite some time is how the calcium i形状 发表于 2025-3-24 03:44:43
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https://doi.org/10.1007/978-3-662-57449-2is far from being completely understood. Therefore further basic research is necessary. With recent developments of optical technologies novel tools to investigate cardiac physiology and pathophysiology became available. They comprise non-linear imaging techniques such as second harmonic generationmedium 发表于 2025-3-24 11:12:39
http://reply.papertrans.cn/23/2209/220811/220811_17.pngCrumple 发表于 2025-3-24 16:24:17
https://doi.org/10.1007/978-3-322-94190-9ent screens. This starts with an optimised culture of human adult cardiomyocytes, allowing culture with diminished dedifferentiation for one week. To allow fluorescence based molecular imaging genetically encoded biosensors need to be expressed in the cardiomyocytes. For transduction of end-differen多产子 发表于 2025-3-24 21:54:18
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https://doi.org/10.1007/978-3-322-85987-7matic because of the autofluorescence or absorption properties of the intracellular milieu. Here, we describe experiments to assess the usability of popular calcium indicators such as Fura-2, Indo-1 and Fluo-4. In our experiments, Fluo-4 turned out to be the preferable indicator because (i) its exci