Madison
发表于 2025-3-21 17:21:32
书目名称CRISPR-Cas Methods影响因子(影响力)<br> http://impactfactor.cn/if/?ISSN=BK0220558<br><br> <br><br>书目名称CRISPR-Cas Methods影响因子(影响力)学科排名<br> http://impactfactor.cn/ifr/?ISSN=BK0220558<br><br> <br><br>书目名称CRISPR-Cas Methods网络公开度<br> http://impactfactor.cn/at/?ISSN=BK0220558<br><br> <br><br>书目名称CRISPR-Cas Methods网络公开度学科排名<br> http://impactfactor.cn/atr/?ISSN=BK0220558<br><br> <br><br>书目名称CRISPR-Cas Methods被引频次<br> http://impactfactor.cn/tc/?ISSN=BK0220558<br><br> <br><br>书目名称CRISPR-Cas Methods被引频次学科排名<br> http://impactfactor.cn/tcr/?ISSN=BK0220558<br><br> <br><br>书目名称CRISPR-Cas Methods年度引用<br> http://impactfactor.cn/ii/?ISSN=BK0220558<br><br> <br><br>书目名称CRISPR-Cas Methods年度引用学科排名<br> http://impactfactor.cn/iir/?ISSN=BK0220558<br><br> <br><br>书目名称CRISPR-Cas Methods读者反馈<br> http://impactfactor.cn/5y/?ISSN=BK0220558<br><br> <br><br>书目名称CRISPR-Cas Methods读者反馈学科排名<br> http://impactfactor.cn/5yr/?ISSN=BK0220558<br><br> <br><br>
戏服
发表于 2025-3-21 20:18:54
Chr. Gutenbrunner,G. Hildebrandtew crop variety. This chapter briefly describes the methods of CRISPR-Cas9 vector construction with single and multiple gene targets in rice. The protocol covers all important steps of target sequence selection, single/multiple-target vector construction, mutation detection, and selection of T-DNA free mutant lines.
乐意
发表于 2025-3-22 00:36:35
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MAZE
发表于 2025-3-22 07:02:47
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下级
发表于 2025-3-22 11:36:04
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墙壁
发表于 2025-3-22 14:41:20
In Vitro Cas9 Cleavage Assay to Check Guide RNA Efficiency,ro method to screen multiple sgRNAs to identify the most suitable one that can efficiently introduce a double-stranded break at a particular genomic target site. This screening method allows a researcher to choose the best one among several online predicted sgRNAs prior to deliver genome editing reagents into live plant or animal cells.
墙壁
发表于 2025-3-22 18:34:18
Generation of Knockout and Fragment Deletion Mutants in Soybean by CRISPR-Cas9,o the CRISPR-Cas9-mediated targeted mutagenesis or large fragment deletions in soybean. Detailed procedures will guide through the essential steps including the design of sgRNAs, construction of CRISPR-Cas9 vectors, .-mediated soybean transformation, and identification of mutant lines.
minimal
发表于 2025-3-23 00:39:44
1949-2448 ation advice from the experts.This second volume provides new and updated methods detailing advancements in CRISPR-Cas technical protocols. Chapters guide readers through protocols on prime editing, base editing, multiplex editing, editing in cell-free extract, in silico analysis of gRNA secondary s
不透气
发表于 2025-3-23 02:38:36
https://doi.org/10.1007/978-3-662-25284-0e, wheat, maize, and tomato, which substantially expands the scope and capabilities of precision plant breeding. Here, we describe a fast and efficient method for construction of prime editing vectors based on Gateway assembly and efficiency assessment of prime editors through transient expression analyses in rice protoplasts.
engrave
发表于 2025-3-23 07:41:14
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