焦虑
发表于 2025-3-28 15:33:30
https://doi.org/10.1007/978-3-642-87580-9ate the effectiveness and specificity of the construct. Our protocol will make these tools accessible to individuals interested in targeted manipulation of DNA methylation in plants for basic research or crop engineering.
抵制
发表于 2025-3-28 21:27:01
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易怒
发表于 2025-3-28 23:28:32
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maintenance
发表于 2025-3-29 07:05:13
In Silico Analysis of gRNA Secondary Structure to Predict Its Efficacy for Plant Genome Editing,acy of the CRISPR-Cas-mediated genome editing are primarily determined by a short sequence known as guide RNA (gRNA). Recent studies have demonstrated that the secondary structure of gRNAs plays a key role in target recognition in CRISPR-Cas-mediated genome editing. Although there are many tools cur
繁荣中国
发表于 2025-3-29 08:00:29
In Vitro Cas9 Cleavage Assay to Check Guide RNA Efficiency, genomes of interest in a wide variety of organisms. The Cas9 endonuclease enzyme is targeted to a specific genomic region by a small single guide RNA (sgRNA). The cleavage efficiency of Cas9 varies greatly from one sgRNA to another sgRNA. Mutagenesis rate of a CRISPR-Cas experiment strongly depends
bronchodilator
发表于 2025-3-29 13:30:23
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宪法没有
发表于 2025-3-29 15:47:51
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加强防卫
发表于 2025-3-29 23:21:18
Rapid Assembly of Multiplex Natural CRISPR Arrays,plexing with CRISPR-Cas9 and its homologs presents various technical challenges, such as very long synthetic targeting arrays and time-consuming assembly. Recently, other CRISPR-associated, single-effector nucleases such as Cas12a have been shown to process their own CRISPR arrays, enabling the use
要素
发表于 2025-3-30 03:58:53
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WITH
发表于 2025-3-30 05:42:33
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