包裹 发表于 2025-3-26 23:52:37
http://reply.papertrans.cn/23/2206/220556/220556_31.png个人长篇演说 发表于 2025-3-27 01:29:32
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Harnessing tRNA for Processing Ability and Promoter Activityengineered tRNAs can be used to process gRNAs from Pol-II transcripts, thus enabling spatial/temporal control of gRNA expression. Here we describe the design, cloning, and testing of tRNA scaffolds for both Pol-III-driven expression of different levels of gRNAs, and for processing gRNAs from Pol-II transcripts.amenity 发表于 2025-3-28 01:02:00
http://reply.papertrans.cn/23/2206/220556/220556_37.pngEndearing 发表于 2025-3-28 05:10:49
https://doi.org/10.1007/978-3-662-25288-8the presence of a user defined inducer. In this chapter, I cover the know-how relating to the design and synthesis of iSBH-sgRNAs, as well as the implementation in mammalian cells of inducible CRISPR-TR strategies based on this technology.Incumbent 发表于 2025-3-28 08:53:19
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