包裹 发表于 2025-3-26 23:52:37

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个人长篇演说 发表于 2025-3-27 01:29:32

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disrupt 发表于 2025-3-27 09:21:09

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意外 发表于 2025-3-27 11:28:08

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FIS 发表于 2025-3-27 15:33:54

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NOTCH 发表于 2025-3-27 19:16:08

Harnessing tRNA for Processing Ability and Promoter Activityengineered tRNAs can be used to process gRNAs from Pol-II transcripts, thus enabling spatial/temporal control of gRNA expression. Here we describe the design, cloning, and testing of tRNA scaffolds for both Pol-III-driven expression of different levels of gRNAs, and for processing gRNAs from Pol-II transcripts.

amenity 发表于 2025-3-28 01:02:00

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Endearing 发表于 2025-3-28 05:10:49

https://doi.org/10.1007/978-3-662-25288-8the presence of a user defined inducer. In this chapter, I cover the know-how relating to the design and synthesis of iSBH-sgRNAs, as well as the implementation in mammalian cells of inducible CRISPR-TR strategies based on this technology.

Incumbent 发表于 2025-3-28 08:53:19

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微尘 发表于 2025-3-28 14:12:22

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查看完整版本: Titlebook: CRISPR Guide RNA Design; Methods and Protocol Tudor A. Fulga,David J. H. F. Knapp,Quentin R. V. Book 2021 Springer Science+Business Media,